Ribonuclease A digestion by proteinase K.
نویسندگان
چکیده
The digestion of ribonuclease A by proteinase K yielded one major degradation product only, which could not be distinguished from ribonuclease S by electrophoretical and immunological methods. This component (ribonuclease K) possessing full catalytic activity was characterized to be (1--20/21--124) ribonuclease A. Combined action of proteinase K and trypsin on ribonuclease A leads to a significant increase of the inactivation rate which may be useful in the isolation of mRNA from polysomes.
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ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Section C, Biosciences
دوره 33 9-10 شماره
صفحات -
تاریخ انتشار 1978